Ontology highlight
ABSTRACT:
SUBMITTER: Winkelman JT
PROVIDER: S-EPMC4561013 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Molecular cell 20150806 5
RNA polymerase binds tightly to DNA to recognize promoters with high specificity but then releases these contacts during the initial stage of transcription. We report a site-specific crosslinking approach to map the DNA path in bacterial transcription intermediates at amino acid and nucleotide resolution. After validating the approach by showing that the DNA path in open complexes (RPO) is the same as in high-resolution X-ray structures, we define the path following substrate addition in "scrunc ...[more]