A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine.
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ABSTRACT:
SUBMITTER: Smith ME
PROVIDER: S-EPMC4563668 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Publications
A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine.
Smith Mark E B ME Caspersen Mikael B MB Robinson Eifion E Morais Maurício M Maruani Antoine A Nunes João P M JP Nicholls Karl K Saxton Malcolm J MJ Caddick Stephen S Baker James R JR Chudasama Vijay V
Organic & biomolecular chemistry 20150625 29
Herein we report the use of bromomaleimides for the construction of stable albumin conjugates via conjugation to its native, single accessible, cysteine followed by hydrolysis. Advantages over the classical maleimide approach are highlighted in terms of quantitative hydrolysis and absence of undesirable retro-Michael deconjugation. ...[more]
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