In silico feedback for in vivo regulation of a gene expression circuit.
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ABSTRACT:
SUBMITTER: Milias-Argeitis A
PROVIDER: S-EPMC4565053 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Publications
In silico feedback for in vivo regulation of a gene expression circuit.
Milias-Argeitis Andreas A Summers Sean S Stewart-Ornstein Jacob J Zuleta Ignacio I Pincus David D El-Samad Hana H Khammash Mustafa M Lygeros John J
Nature biotechnology 20111106 12
We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes wh ...[more]
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