Ontology highlight
ABSTRACT:
SUBMITTER: Kian D
PROVIDER: S-EPMC4566500 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Kian Danielle D Lancheros César Armando Contreras CA Damiani Igor Alexandre Campos IA Fernandes Tamiris Zanforlin Olmos TZ Pinge-Filho Phileno P Santos Márcia Regina Machado dos MR Silveira José Franco da JF Nakamura Celso Vataru CV Silva João Santana da JS Yamada-Ogatta Sueli Fumie SF Yamauchi Lucy Megumi LM
The Korean journal of parasitology 20150825 4
This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigot ...[more]