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High-resolution analysis of the human T-cell receptor repertoire.

ABSTRACT: Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies TCR ?- and ?-chain diversity without sequence-associated or quantitative restrictions in healthy and diseased conditions. TCR-LA-MC PCR identifies convergent recombination events, classifies different stages of cutaneous T-cell lymphoma in vivo and demonstrates TCR reactivation after in vitro cytomegalovirus stimulation. TCR-LA-MC PCR allows ultra-deep data access to both physiological TCR diversity and mechanisms influencing clonality in all clinical settings with restricted or distorted TCR repertoires.

SUBMITTER: Ruggiero E

PROVIDER: S-EPMC4569693 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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High-resolution analysis of the human T-cell receptor repertoire.

Ruggiero Eliana E Nicolay Jan P JP Fronza Raffaele R Arens Anne A Paruzynski Anna A Nowrouzi Ali A Ürenden Gökçe G Lulay Christina C Schneider Sven S Goerdt Sergij S Glimm Hanno H Krammer Peter H PH Schmidt Manfred M von Kalle Christof C

Nature communications 20150901

Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies TCR α- and β-chain diversity without sequence-associated or quantitative restrictions in healthy and diseased conditions. TCR-LA-MC PCR identifies convergent recombination events, classifies different stages of cutaneous T-cell lymphoma in vivo and demonstrates T ...[more]

PMID: 26324409

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