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Lichen Secondary Metabolite, Physciosporin, Inhibits Lung Cancer Cell Motility.

ABSTRACT: Lichens produce various unique chemicals that can be used for pharmaceutical purposes. To screen for novel lichen secondary metabolites showing inhibitory activity against lung cancer cell motility, we tested acetone extracts of 13 lichen samples collected in Chile. Physciosporin, isolated from Pseudocyphellaria coriacea (Hook f. & Taylor) D.J. Galloway & P. James, was identified as an effective compound and showed significant inhibitory activity in migration and invasion assays against human lung cancer cells. Physciosporin treatment reduced both protein and mRNA levels of N-cadherin with concomitant decreases in the levels of epithelial-mesenchymal transition markers such as snail and twist. Physciosporin also suppressed KITENIN (KAI1 C-terminal interacting tetraspanin)-mediated AP-1 activity in both the absence and presence of epidermal growth factor stimulation. Quantitative real-time PCR analysis showed that the expression of the metastasis suppressor gene, KAI1, was increased while that of the metastasis enhancer gene, KITENIN, was dramatically decreased by physciosporin. Particularly, the activity of 3'-untranslated region of KITENIN was decreased by physciosporin. Moreover, Cdc42 and Rac1 activities were decreased by physciosporin. These results demonstrated that the lichen secondary metabolite, physciosporin, inhibits lung cancer cell motility through novel mechanisms of action.

SUBMITTER: Yang Y

PROVIDER: S-EPMC4570789 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Lichen Secondary Metabolite, Physciosporin, Inhibits Lung Cancer Cell Motility.

Yang Yi Y Park So-Yeon SY Nguyen Thanh Thi TT Yu Young Hyun YH Nguyen Tru Van TV Sun Eun Gene EG Udeni Jayalal J Jeong Min-Hye MH Pereira Iris I Moon Cheol C Ha Hyung-Ho HH Kim Kyung Keun KK Hur Jae-Seoun JS Kim Hangun H

PloS one 20150915 9

Lichens produce various unique chemicals that can be used for pharmaceutical purposes. To screen for novel lichen secondary metabolites showing inhibitory activity against lung cancer cell motility, we tested acetone extracts of 13 lichen samples collected in Chile. Physciosporin, isolated from Pseudocyphellaria coriacea (Hook f. & Taylor) D.J. Galloway & P. James, was identified as an effective compound and showed significant inhibitory activity in migration and invasion assays against human lu ...[more]

PMID: 26371759

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Dataset Information

Lichen Secondary Metabolite, Physciosporin, Inhibits Lung Cancer Cell Motility.

Publications

Lichen Secondary Metabolite, Physciosporin, Inhibits Lung Cancer Cell Motility.

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