Ontology highlight
ABSTRACT:
SUBMITTER: Jusuk I
PROVIDER: S-EPMC4571581 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Jusuk Ija I Vietz Carolin C Raab Mario M Dammeyer Thorben T Tinnefeld Philip P
Scientific reports 20150916
Photostability is one of the crucial properties of a fluorophore which strongly influences the quality of single molecule-based super-resolution imaging. Enhanced yellow fluorescent protein (eYFP) is one of the most widely used versions of fluorescent proteins in modern cell biology exhibiting fast intrinsic blinking and reversible photoactivation by UV light. Here, we developed an assay for studying photostabilization of single eYFP molecules with respect to fast blinking and demonstrated a 6-f ...[more]