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Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy.


ABSTRACT: Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles and plays a vital role in studying molecular structures and dynamics of bio-complexes. However, current techniques remain difficult to resolve the dipole assemblies on subcellular structures and their dynamics in living cells at super-resolution level. Here we report polarized structured illumination microscopy (pSIM), which achieves super-resolution imaging of dipoles by interpreting the dipoles in spatio-angular hyperspace. We demonstrate the application of pSIM on a series of biological filamentous systems, such as cytoskeleton networks and ?-DNA, and report the dynamics of short actin sliding across a myosin-coated surface. Further, pSIM reveals the side-by-side organization of the actin ring structures in the membrane-associated periodic skeleton of hippocampal neurons and images the dipole dynamics of green fluorescent protein-labeled microtubules in live U2OS cells. pSIM applies directly to a large variety of commercial and home-built SIM systems with various imaging modality.

SUBMITTER: Zhanghao K 

PROVIDER: S-EPMC6795901 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy.

Zhanghao Karl K   Chen Xingye X   Liu Wenhui W   Li Meiqi M   Liu Yiqiong Y   Wang Yiming Y   Luo Sha S   Wang Xiao X   Shan Chunyan C   Xie Hao H   Gao Juntao J   Chen Xiaowei X   Jin Dayong D   Li Xiangdong X   Zhang Yan Y   Dai Qionghai Q   Xi Peng P  

Nature communications 20191016 1


Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles and plays a vital role in studying molecular structures and dynamics of bio-complexes. However, current techniques remain difficult to resolve the dipole assemblies on subcellular structures and their dynamics in living cells at super-resolution level. Here we report polarized structured illumination microscopy (pSIM), which achieves super-resolution imaging of dipoles by interpreting the dipole  ...[more]

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