Ontology highlight
ABSTRACT:
SUBMITTER: Li S
PROVIDER: S-EPMC4576194 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Li Su S Feng Shuo S Wang Jing-Han JH He Wen-Rui WR Qin Hua-Yang HY Dong Hong H Li Lian-Feng LF Yu Shao-Xiong SX Li Yongfeng Y Qiu Hua-Ji HJ
Viruses 20150810 8
The NS5A protein of classical swine fever virus (CSFV) is involved in the RNA synthesis and viral replication. However, the NS5A-interacting cellular proteins engaged in the CSFV replication are poorly defined. Using yeast two-hybrid screen, the eukaryotic elongation factor 1A (eEF1A) was identified to be an NS5A-binding partner. The NS5A-eEF1A interaction was confirmed by coimmunoprecipitation, glutathione S-transferase (GST) pulldown and laser confocal microscopy assays. The domain I of eEF1A ...[more]