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Going Viral with Fluorescent Proteins.

ABSTRACT: Many longstanding questions about dynamics of virus-cell interactions can be answered by combining fluorescence imaging techniques with fluorescent protein (FP) tagging strategies. Successfully creating a FP fusion with a cellular or viral protein of interest first requires selecting the appropriate FP. However, while viral architecture and cellular localization often dictate the suitability of a FP, a FP's chemical and physical properties must also be considered. Here, we discuss the challenges of and offer suggestions for identifying the optimal FPs for studying the cell biology of viruses.

SUBMITTER: Costantini LM

PROVIDER: S-EPMC4577878 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Going Viral with Fluorescent Proteins.

Costantini Lindsey M LM Snapp Erik L EL

Journal of virology 20150722 19

Many longstanding questions about dynamics of virus-cell interactions can be answered by combining fluorescence imaging techniques with fluorescent protein (FP) tagging strategies. Successfully creating a FP fusion with a cellular or viral protein of interest first requires selecting the appropriate FP. However, while viral architecture and cellular localization often dictate the suitability of a FP, a FP's chemical and physical properties must also be considered. Here, we discuss the challenges ...[more]

PMID: 26202231

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