Expression of Protein Kinase C Isoforms in Pancreatic Islets and Liver of Male Goto-Kakizaki Rats, a Model of Type 2 Diabetes.

ABSTRACT: Protein kinase C (PKC) is a family of protein kinases controlling protein phosphorylation and playing important roles in the regulation of metabolism. We have investigated expression levels of PKC isoforms in pancreatic islets and liver of diabetic Goto-Kakizaki (GK) rats with and without insulin treatment to evaluate their association with glucose homeostasis. mRNA and protein expression levels of PKC isoforms were assessed in pancreatic islets and liver of Wistar rats and GK rats with or without insulin treatment. PKC? and PKC? mRNA expressions were down-regulated in islets of GK compared with Wistar rats. PKC? and phosphorylated PKC? (p-PKC?) protein expressions were decreased in islets of GK compared with insulin-treated GK and Wistar rats. PKC? protein expression in islets was reduced in GK and insulin-treated GK compared with Wistar rats, but p-PKC? was decreased only in GK rats. Islet PKC? mRNA and protein expressions were lower in GK compared with insulin-treated GK and Wistar rats. In liver, PKC? and PKC? mRNA expressions were decreased in both GK and insulin-treated GK compared with Wistar rats. Hepatic PKC? protein expression was diminished in both GK rats with and without insulin treatment compared with Wistar rats. Hepatic PKC? mRNA expression was down-regulated in insulin-treated GK compared with GK and Wistar rats. PKC?, PKC?, and p-PKC? expressions were secondary to hyperglycaemia in GK rat islets. Hepatic PKC? and PKC? mRNA expressions were primarily linked to hyperglycaemia. Additionally, hepatic PKC? mRNA expression could be under control of insulin.

SUBMITTER: Seed Ahmed M 

PROVIDER: S-EPMC4580567 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature