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Functionalized Polymer Microgel Particles Enable Customizable Production of Label-Free Sensor Arrays.

ABSTRACT: Probe molecule immobilization onto surfaces is a critical step in the production of many analytical devices, including labeled and label-free microarrays. New methods to increase the density and uniformity of probe deposition have the potential to significantly enhance the ultimate limits of detection and reproducibility. Hydrogel-based materials have been employed in the past to provide a 3D protein-friendly surface for deposition of antibodies and nucleic acids. However, these methods are susceptible to variation during polymerization of the hydrogel scaffold and provide limited opportunities for tuning deposition parameters on an antibody-by-antibody basis. In this work, a versatile hydrogel nanoparticle deposition method was developed for the production of label-free microarrays and tested in the context of antibody-antigen binding. Poly(N-isopropylacrylamide) nanoparticles (PNIPAM) were conjugated to antibodies using an avidin/biotin system and deposited onto surfaces using a noncontact printing system. After drying, these gel spots formed uniform and thin layers <10 nm in height. The conjugates were characterized with dynamic light scattering, scanning electron microscopy, and atomic force microscopy. We tested this format in the context of tumor necrosis factor-alpha (TNF-?) detection via arrayed imaging reflectometry (AIR), a label-free protein microarray method. This method of probe molecule deposition should be generally useful in the production of microarrays for label-free detection.

SUBMITTER: Lifson MA

PROVIDER: S-EPMC4581722 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Functionalized Polymer Microgel Particles Enable Customizable Production of Label-Free Sensor Arrays.

Lifson Mark A MA Carter Jared A JA Miller Benjamin L BL

Analytical chemistry 20150715 15

Probe molecule immobilization onto surfaces is a critical step in the production of many analytical devices, including labeled and label-free microarrays. New methods to increase the density and uniformity of probe deposition have the potential to significantly enhance the ultimate limits of detection and reproducibility. Hydrogel-based materials have been employed in the past to provide a 3D protein-friendly surface for deposition of antibodies and nucleic acids. However, these methods are susc ...[more]

PMID: 26140413

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Functionalized Polymer Microgel Particles Enable Customizable Production of Label-Free Sensor Arrays.

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Functionalized Polymer Microgel Particles Enable Customizable Production of Label-Free Sensor Arrays.

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