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A Nanocrystal-based Ratiometric pH Sensor for Natural pH Ranges.

ABSTRACT: A ratiometric fluorescent pH sensor based on CdSe/CdZnS nanocrystal quantum dots (NCs) has been designed for biological pH ranges. The construct is formed from the conjugation of a pH dye (SNARF) to NCs coated with a poly(amido amine) (PAMAM) dendrimer. The sensor exhibits a well-resolved ratio response at pH values between 6 and 8 under linear or two-photon excitation, and in the presence of a 4% bovine serum albumin (BSA) solution.

SUBMITTER: Somers RC

PROVIDER: S-EPMC4582792 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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A Nanocrystal-based Ratiometric pH Sensor for Natural pH Ranges.

Somers Rebecca C RC Lanning Ryan M RM Snee Preston T PT Greytak Andrew B AB Jain Rakesh K RK Bawendi Moungi G MG Nocera Daniel G DG

Chemical science 20120101 10

A ratiometric fluorescent pH sensor based on CdSe/CdZnS nanocrystal quantum dots (NCs) has been designed for biological pH ranges. The construct is formed from the conjugation of a pH dye (SNARF) to NCs coated with a poly(amido amine) (PAMAM) dendrimer. The sensor exhibits a well-resolved ratio response at pH values between 6 and 8 under linear or two-photon excitation, and in the presence of a 4% bovine serum albumin (BSA) solution. ...[more]

PMID: 26413260

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