Multiplexed 3D FRET imaging in deep tissue of live embryos.
Ontology highlight
ABSTRACT:
SUBMITTER: Zhao M
PROVIDER: S-EPMC4585674 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Publications
Multiplexed 3D FRET imaging in deep tissue of live embryos.
Zhao Ming M Wan Xiaoyang X Li Yu Y Zhou Weibin W Peng Leilei L
Scientific reports 20150921
Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos with high spatial resolution. The method simultaneously images fluorescence lifetimes in 3D with multiple excitation lasers. Through quantitative analysis of ...[more]
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