Multiplexed aberration measurement for deep tissue imaging in vivo.
Ontology highlight
ABSTRACT:
SUBMITTER: Wang C
PROVIDER: S-EPMC4180771 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Multiplexed aberration measurement for deep tissue imaging in vivo.
Wang Chen C Liu Rui R Milkie Daniel E DE Sun Wenzhi W Tan Zhongchao Z Kerlin Aaron A Chen Tsai-Wen TW Kim Douglas S DS Ji Na N
Nature methods 20140817 10
We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging vol ...[more]
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