Ontology highlight
ABSTRACT:
SUBMITTER: Bodega G
PROVIDER: S-EPMC4589356 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Bodega Guillermo G Segura Berta B Ciordia Sergio S Mena María Del Carmen Mdel C López-Fernández Luis Andrés LA García María Isabel MI Trabado Isabel I Suárez Isabel I
PloS one 20150930 9
Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days. Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase. The DNA content was reduced in the treated cells, and BrdU incorporation diminished. However, neither ammonia nor ammonia plus glutamine had any effect on DNA polymerase activity. iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heteroc ...[more]