Titanium Dioxide Nanoparticles (TiO?) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection.
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ABSTRACT: We demonstrate for the first time, the development of titanium dioxide nanoparticles (TiO?) quenching based aptasensing platform for detection of target molecules. TiO? quench the fluorescence of FAM-labeled aptamer (fluorescein labeled aptamer) upon the non-covalent adsorption of fluorescent labeled aptamer on TiO? surface. When OTA interacts with the aptamer, it induced aptamer G-quadruplex complex formation, weakens the interaction between FAM-labeled aptamer and TiO?, resulting in fluorescence recovery. As a proof of concept, an assay was employed for detection of Ochratoxin A (OTA). At optimized experimental condition, the obtained limit of detection (LOD) was 1.5 nM with a good linearity in the range 1.5 nM to 1.0 µM for OTA. The obtained results showed the high selectivity of assay towards OTA without interference to structurally similar analogue Ochratoxin B (OTB). The developed aptamer assay was evaluated for detection of OTA in beer sample and recoveries were recorded in the range from 94.30%-99.20%. Analytical figures of the merits of the developed aptasensing platform confirmed its applicability to real samples analysis. However, this is a generic aptasensing platform and can be extended for detection of other toxins or target analyte.
SUBMITTER: Sharma A
PROVIDER: S-EPMC4591649 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
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