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Mediatorless Impedance Studies with Titanium Dioxide Conjugated Gold Nanoparticles for Hydrogen Peroxide Detection.


ABSTRACT: An impedimetric-based biosensor constructed using gold nanoparticles (AuNP) entrapped within titanium dioxide (TiO?) particles for hydrogen peroxide (H?O?) detection is the main feature of this research. The matrix of the biosensor employed the surface of TiO?, which was previously modified with an amine terminal group using 3-Aminopropyltriethoxysilane (APTS) at a low temperature to create a ready to immobilise surface for the biosensor application. Hemoglobin (Hb), which exhibits peroxidase-like activity, was used as the bioreceptor in the biosensor to detect H?O? in solution. The analysis was carried out using an alternative impedance method, in which the biosensor exhibited a wide linear range response between 1 × 10-4 M and 1.5 × 10-2 M and a limit of detection (LOD) of 1 × 10-5 M without a redox mediator.

SUBMITTER: Abdul Halim NH 

PROVIDER: S-EPMC5618044 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Mediatorless Impedance Studies with Titanium Dioxide Conjugated Gold Nanoparticles for Hydrogen Peroxide Detection.

Abdul Halim Nur Hamidah NH   Lee Yook Heng YH   Marugan Radha Swathe Priya Malon RSPM   Hashim Uda U  

Biosensors 20170918 3


An impedimetric-based biosensor constructed using gold nanoparticles (AuNP) entrapped within titanium dioxide (TiO₂) particles for hydrogen peroxide (H₂O₂) detection is the main feature of this research. The matrix of the biosensor employed the surface of TiO₂, which was previously modified with an amine terminal group using 3-Aminopropyltriethoxysilane (APTS) at a low temperature to create a ready to immobilise surface for the biosensor application. Hemoglobin (Hb), which exhibits peroxidase-li  ...[more]

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