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The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation.


ABSTRACT: The formation of the mitotic spindle is a complex process that requires massive cellular reorganization. Regulation by mitotic kinases controls this entire process. One of these mitotic controllers is Aurora A kinase, which is itself highly regulated. In this study, we show that the nuclear pore protein ALADIN is a novel spatial regulator of Aurora A. Without ALADIN, Aurora A spreads from centrosomes onto spindle microtubules, which affects the distribution of a subset of microtubule regulators and slows spindle assembly and chromosome alignment. ALADIN interacts with inactive Aurora A and is recruited to the spindle pole after Aurora A inhibition. Of interest, mutations in ALADIN cause triple A syndrome. We find that some of the mitotic phenotypes that we observe after ALADIN depletion also occur in cells from triple A syndrome patients, which raises the possibility that mitotic errors may underlie part of the etiology of this syndrome.

SUBMITTER: Carvalhal S 

PROVIDER: S-EPMC4591688 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation.

Carvalhal Sara S   Ribeiro Susana Abreu SA   Arocena Miguel M   Kasciukovic Taciana T   Temme Achim A   Koehler Katrin K   Huebner Angela A   Griffis Eric R ER  

Molecular biology of the cell 20150805 19


The formation of the mitotic spindle is a complex process that requires massive cellular reorganization. Regulation by mitotic kinases controls this entire process. One of these mitotic controllers is Aurora A kinase, which is itself highly regulated. In this study, we show that the nuclear pore protein ALADIN is a novel spatial regulator of Aurora A. Without ALADIN, Aurora A spreads from centrosomes onto spindle microtubules, which affects the distribution of a subset of microtubule regulators  ...[more]

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