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Rapid low dose electron tomography using a direct electron detection camera.


ABSTRACT: We demonstrate the ability to record a tomographic tilt series containing 3487 images in only 3.5 s by using a direct electron detector in a transmission electron microscope. The electron dose is lower by at least one order of magnitude when compared with that used to record a conventional tilt series of fewer than 100 images in 15-60 minutes and the overall signal-to-noise ratio is greater than 4. Our results, which are illustrated for an inorganic nanotube, are important for ultra-low-dose electron tomography of electron-beam-sensitive specimens and real-time dynamic electron tomography of nanoscale objects with sub-ms temporal resolution.

SUBMITTER: Migunov V 

PROVIDER: S-EPMC4592966 | biostudies-literature |

REPOSITORIES: biostudies-literature

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