Ontology highlight
ABSTRACT:
SUBMITTER: Pfeffer S
PROVIDER: S-EPMC4598622 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Pfeffer Stefan S Burbaum Laura L Unverdorben Pia P Pech Markus M Chen Yuxiang Y Zimmermann Richard R Beckmann Roland R Förster Friedrich F
Nature communications 20150928
In mammalian cells, secretory and membrane proteins are translocated across or inserted into the endoplasmic reticulum (ER) membrane by the universally conserved protein-conducting channel Sec61, which has been structurally studied in isolated, detergent-solubilized states. Here we structurally and functionally characterize native, non-solubilized ribosome-Sec61 complexes on rough ER vesicles using cryo-electron tomography and ribosome profiling. Surprisingly, the 9-Å resolution subtomogram aver ...[more]