Ontology highlight
ABSTRACT:
SUBMITTER: Haßdenteufel S
PROVIDER: S-EPMC5946456 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Haßdenteufel Sarah S Johnson Nicholas N Paton Adrienne W AW Paton James C JC High Stephen S Zimmermann Richard R
Cell reports 20180501 5
Protein transport into the mammalian endoplasmic reticulum (ER) is mediated by the heterotrimeric Sec61 channel. The signal recognition particle (SRP) and TRC systems and Sec62 have all been characterized as membrane-targeting components for small presecretory proteins, whereas Sec63 and the lumenal chaperone BiP act as auxiliary translocation components. Here, we report the transport requirements of two natural, small presecretory proteins and engineered variants using semipermeabilized human c ...[more]