Ontology highlight
ABSTRACT:
SUBMITTER: Lu R
PROVIDER: S-EPMC4600597 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Lu Rui R Schmitz Werner W Sampson Nicole S NS
Biochemistry 20150911 37
Metabolism of cholesterol by Mycobacterium tuberculosis (Mtb) contributes to its pathogenesis. We show that ChsE4-ChsE5 (Rv3504/Rv3505) specifically catalyzes dehydrogenation of the (25S)-3-oxo-cholest-4-en-26-oyl-CoA diastereomer in cholesterol side chain β-oxidation. Thus, a dichotomy between the supply of both 25R and 25S metabolic precursors by upstream cytochrome P450s and the substrate stereospecificity of ChsE4-ChsE5 exists. We reconcile the dilemma of 25R metabolite production by demonst ...[more]