Ontology highlight
ABSTRACT:
SUBMITTER: Sokoll S
PROVIDER: S-EPMC4601043 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Sokoll Stefan S Prokazov Yury Y Hanses Magnus M Biermann Barbara B Tönnies Klaus K Heine Martin M
Biophysical journal 20151001 7
Observation of molecular dynamics is often biased by the optical very heterogeneous environment of cells and complex tissue. Here, we have designed an algorithm that facilitates molecular dynamic analyses within brain slices. We adjust fast astigmatism-based three-dimensional single-particle tracking techniques to depth-dependent optical aberrations induced by the refractive index mismatch so that they are applicable to complex samples. In contrast to existing techniques, our online calibration ...[more]