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Basophils are not the key antigen-presenting cells in allergic patients.

ABSTRACT: Recent data obtained in mouse models have initiated a controversy whether basophils are the key antigen-presenting cells (APCs) in allergy. Here, we investigate whether basophils are of importance for the presentation of allergen and the induction of T cell proliferation in allergic patients.T cells, basophils, and APCs depleted of basophils were purified from allergic patients. Co-culture systems based on purified major allergens were established to study allergen-specific T cell responses using proliferation assays.Only co-cultures of T cells with APCs depleted of basophils but not with basophils proliferated in response to allergen. Even addition of IL-3 to T cell-basophil co-cultures failed to induce allergen-specific T cell proliferation.Our data demonstrate by classical in vitro proliferation assays that basophils are not key antigen-presenting cells that promote T cell proliferation in secondary immune responses to allergen in allergic patients.

SUBMITTER: Eckl-Dorna J

PROVIDER: S-EPMC4601523 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Basophils are not the key antigen-presenting cells in allergic patients.

Eckl-Dorna J J Ellinger A A Blatt K K Ghanim V V Steiner I I Pavelka M M Valent P P Valenta R R Niederberger V V

Allergy 20120216 5

<h4>Background</h4>Recent data obtained in mouse models have initiated a controversy whether basophils are the key antigen-presenting cells (APCs) in allergy. Here, we investigate whether basophils are of importance for the presentation of allergen and the induction of T cell proliferation in allergic patients.<h4>Methods</h4>T cells, basophils, and APCs depleted of basophils were purified from allergic patients. Co-culture systems based on purified major allergens were established to study alle ...[more]

PMID: 22335568

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