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?-synuclein assemblies sequester neuronal ?3-Na+/K+-ATPase and impair Na+ gradient.


ABSTRACT: Extracellular ?-synuclein (?-syn) assemblies can be up-taken by neurons; however, their interaction with the plasma membrane and proteins has not been studied specifically. Here we demonstrate that ?-syn assemblies form clusters within the plasma membrane of neurons. Using a proteomic-based approach, we identify the ?3-subunit of Na+/K+-ATPase (NKA) as a cell surface partner of ?-syn assemblies. The interaction strength depended on the state of ?-syn, fibrils being the strongest, oligomers weak, and monomers none. Mutations within the neuron-specific ?3-subunit are linked to rapid-onset dystonia Parkinsonism (RDP) and alternating hemiplegia of childhood (AHC). We show that freely diffusing ?3-NKA are trapped within ?-syn clusters resulting in ?3-NKA redistribution and formation of larger nanoclusters. This creates regions within the plasma membrane with reduced local densities of ?3-NKA, thereby decreasing the efficiency of Na+ extrusion following stimulus. Thus, interactions of ?3-NKA with extracellular ?-syn assemblies reduce its pumping activity as its mutations in RDP/AHC.

SUBMITTER: Shrivastava AN 

PROVIDER: S-EPMC4601662 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Extracellular α-synuclein (α-syn) assemblies can be up-taken by neurons; however, their interaction with the plasma membrane and proteins has not been studied specifically. Here we demonstrate that α-syn assemblies form clusters within the plasma membrane of neurons. Using a proteomic-based approach, we identify the α3-subunit of Na+/K+-ATPase (NKA) as a cell surface partner of α-syn assemblies. The interaction strength depended on the state of α-syn, fibrils being the strongest, oligomers weak,  ...[more]

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