Ontology highlight
ABSTRACT:
SUBMITTER: Tamaru D
PROVIDER: S-EPMC6191716 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Tamaru Daichi D Amikura Kazuaki K Shimizu Yoshihiro Y Nierhaus Knud H KH Ueda Takuya T
RNA (New York, N.Y.) 20180803 11
Reconstitution of ribosomes in vitro from individual ribosomal proteins provides a powerful tool for understanding the ribosome assembly process including the sequential incorporation of ribosomal proteins. However, conventional assembly methods require high-salt conditions for efficient ribosome assembly. In this study, we reconstituted 30S ribosomal subunits from individually purified ribosomal proteins in the presence of ribosome biogenesis factors. In this system, two GTPases (Era and YjeQ) ...[more]