Ontology highlight
ABSTRACT:
SUBMITTER: Lin Q
PROVIDER: S-EPMC4609996 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Lin Qiuhui Q Qi Hao H Wu Yi Y Yuan Yingjin Y
Scientific reports 20151019
Rearrangement of genomic DNA elements in a dynamic controlled fashion is a fundamental challenge. Site-specific DNA recombinases have been tamed as a powerful tool in genome editing. Here, we reported a DNA element rearrangement on the basis of a pairwise orthogonal recombination system which is comprised of two site-specific recombinases of Vika/vox and Cre/loxp in yeast Saccharomyces Creevisiae. Taking the advantage of the robust pairwise orthogonality, we showed that multi gene elements could ...[more]