Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein.
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ABSTRACT:
SUBMITTER: Bogdanov IV
PROVIDER: S-EPMC4610166 | biostudies-literature | 2015 Jul-Sep
REPOSITORIES: biostudies-literature
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Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein.
Bogdanov I V IV Finkina E I EI Balandin S V SV Melnikova D N DN Stukacheva E A EA Ovchinnikova T V TV
Acta naturae 20150701 3
The recombinant isoforms Lc-LTP1 and Lc-LTP3 of the lentil lipid transfer protein were overexpressed in E. coli cells. It was confirmed that both proteins are stabilized by four disulfide bonds and characterized by a high proportion of the α-helical structure. It was found that Lc-LTP1 and Lc-LTP3 possess antimicrobial activity and can bind fatty acids. Both isoforms have the ability to bind specific IgE from sera of patients with food allergies, which recognize similar epitopes of the major pea ...[more]
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