PLZF Controls the Development of Fetal-Derived IL-17+V?6+ ?? T Cells.

ABSTRACT: Expression of promyelocytic leukemia zinc finger (PLZF) protein directs the effector differentiation of invariant NKT (iNKT) cells and IL-4(+) ?? NKT cells. In this study, we show that PLZF is also required for the development and function of IL-17(+) ?? T cells. We observed that PLZF is expressed in fetal-derived invariant V?5(+) and V?6(+) ?? T cells, which secrete IFN-? and IL-17, respectively. PLZF deficiency specifically affected the effector differentiation of V?6(+) cells, leading to reduced numbers of mature CD27(-)CD44(+) phenotype capable of secreting IL-17. Although PLZF was not required for V?5(+) ?? T cells to develop, when these cells were reprogrammed into IL-17-secreting cells in Skint-1 mutant mice, they required PLZF for their effector maturation, similarly to V?6(+) ?? T cells. The impaired effector differentiation of PLZF-deficient V?6(+) ?? T cells was not due to increased apoptosis and it was related to reduced proliferation of immature CD27(+)CD44(-) V?6(+) ?? T cells, which was required for their differentiation into mature CD27(-)CD44(+) IL-17-secreting cells. Thus, the present study identifies that PLZF function is not restricted to NKT or IL-4(+) T cells, but it also controls the development of IL-17(+) ?? T cells.

SUBMITTER: Lu Y 

PROVIDER: S-EPMC4610873 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature