Ontology highlight
ABSTRACT:
SUBMITTER: Plummer AM
PROVIDER: S-EPMC4613867 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Plummer Ashlee M AM Fleming Karen G KG
Biochemistry 20151001 39
β-Barrel assembly machinery protein A (BamA) plays a critical role in the biogenesis of outer membrane proteins (OMPs); however, a mechanistic understanding of its function is lacking. Here, we report an in vitro assay that investigates whether the mechanism of BamA-catalyzed OMP folding is stoichiometric or catalytic. We found that BamA accelerates the folding of OMPs in vitro via a catalytic mechanism, similar to the activity of the full multiprotein β-barrel assembly machinery (BAM) complex i ...[more]