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An Xist-activating antisense RNA required for X-chromosome inactivation.


ABSTRACT: The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.

SUBMITTER: Sarkar MK 

PROVIDER: S-EPMC4616153 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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An Xist-activating antisense RNA required for X-chromosome inactivation.

Sarkar Mrinal K MK   Gayen Srimonta S   Kumar Surinder S   Maclary Emily E   Buttigieg Emily E   Hinten Michael M   Kumari Archana A   Harris Clair C   Sado Takashi T   Kalantry Sundeep S  

Nature communications 20151019


The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-codi  ...[more]

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