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D-repeat in the XIST gene is required for X chromosome inactivation.

ABSTRACT: XIST is a long non-coding RNA, which expressed exclusively from the inactive X chromosome. Although it has been revealed that the A-repeat contributes to the X chromosome inactivation (X-inactivation), the role of the longest D-repeat has not yet been investigated. Here, a sgRNA directed CRISPR/Cas9 system which have multiple target sites within repeat D of XIST, were used to generate D-repeat deletion and studied its roles on X-inactivation. The results showed that the deletion of D-repeat caused a significantly decreased expression of XIST, and up regulated expression of X-linked genes, suggesting that the D-repeat may play an important role in the regulation of XIST expression and silencing of the X-linked genes, which could provide a new idea in the molecular mechanisms of X-inactivation.

SUBMITTER: Lv Q

PROVIDER: S-EPMC4829313 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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D-repeat in the XIST gene is required for X chromosome inactivation.

Lv Qingyan Q Yuan Lin L Song Yuning Y Sui Tingting T Li Zhanjun Z Lai Liangxue L

RNA biology 20160101 2

XIST is a long non-coding RNA, which expressed exclusively from the inactive X chromosome. Although it has been revealed that the A-repeat contributes to the X chromosome inactivation (X-inactivation), the role of the longest D-repeat has not yet been investigated. Here, a sgRNA directed CRISPR/Cas9 system which have multiple target sites within repeat D of XIST, were used to generate D-repeat deletion and studied its roles on X-inactivation. The results showed that the deletion of D-repeat caus ...[more]

PMID: 26786668

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