Ontology highlight
ABSTRACT:
SUBMITTER: Tempel W
PROVIDER: S-EPMC4617357 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Tempel Wolfram W Grabovec Irina I MacKenzie Farrell F Dichenko Yaroslav V YV Usanov Sergey A SA Gilep Andrei A AA Park Hee-Won HW Strushkevich Natallia N
Journal of lipid research 20140613 9
Hepatic conversion to bile acids is a major elimination route for cholesterol in mammals. CYP7A1 catalyzes the first and rate-limiting step in classic bile acid biosynthesis, converting cholesterol to 7α-hydroxycholesterol. To identify the structural determinants that govern the stereospecific hydroxylation of cholesterol, we solved the crystal structure of CYP7A1 in the ligand-free state. The structure-based mutation T104L in the B' helix, corresponding to the nonpolar residue of CYP7B1, was us ...[more]