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Inducible gene expression system by 3-hydroxypropionic acid.


ABSTRACT: BACKGROUND:3-Hydroxypropionic acid (3-HP) is an important platform chemical that boasts a variety of industrial applications. Gene expression systems inducible by 3-HP, if available, are of great utility for optimization of the pathways of 3-HP production and excretion. RESULTS:Here we report the presence of unique inducible gene expression systems in Pseudomonas denitrificans and other microorganisms. In P. denitrificans, transcription of three genes (hpdH, mmsA and hbdH-4) involved in 3-HP degradation was upregulated by 3-HP by the action of a transcriptional regulator protein, LysR, and a cis-acting regulatory site for LysR binding. Similar inducible systems having an LysR transcriptional regulator were identified in other microorganisms that also could degrade 3-HP. A docking study showed that the 3-HP binding pocket is located between the N-terminal helix-turn-helix motif and the C-terminal cofactor-binding domain. CONCLUSIONS:This LysR-regulated 3-HP-inducible system should prove useful for control of the level of gene expression in response to 3-HP.

SUBMITTER: Zhou S 

PROVIDER: S-EPMC4617489 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Inducible gene expression system by 3-hydroxypropionic acid.

Zhou Shengfang S   Ainala Satish Kumar SK   Seol Eunhee E   Nguyen Trinh Thi TT   Park Sunghoon S  

Biotechnology for biofuels 20151020


<h4>Background</h4>3-Hydroxypropionic acid (3-HP) is an important platform chemical that boasts a variety of industrial applications. Gene expression systems inducible by 3-HP, if available, are of great utility for optimization of the pathways of 3-HP production and excretion.<h4>Results</h4>Here we report the presence of unique inducible gene expression systems in Pseudomonas denitrificans and other microorganisms. In P. denitrificans, transcription of three genes (hpdH, mmsA and hbdH-4) invol  ...[more]

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