Ontology highlight
ABSTRACT:
SUBMITTER: Percival-Alwyn JL
PROVIDER: S-EPMC4622073 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Percival-Alwyn Jennifer L JL England Elizabeth E Kemp Benjamin B Rapley Laura L Davis Nicola H E NH McCarthy Grant R GR Majithiya Jayesh B JB Corkill Dominic J DJ Welsted Sarah S Minton Kevin K Cohen E Suzanne ES Robinson Matthew J MJ Dobson Claire C Wilkinson Trevor C I TC Vaughan Tristan J TJ Groves Maria A T MA Tigue Natalie J NJ
mAbs 20150101 1
Immunization of mice or rats with a "non-self" protein is a commonly used method to obtain monoclonal antibodies, and relies on the immune system's ability to recognize the immunogen as foreign. Immunization of an antigen with 100% identity to the endogenous protein, however, will not elicit a robust immune response. To develop antibodies to mouse proteins, we focused on the potential for breaking such immune tolerance by genetically fusing two independent T-cell epitope-containing sequences (fr ...[more]