ABSTRACT: To better understand how ?-cells respond to proinflammatory cytokines we mapped the locations of histone 3 lysine 4 monomethylation (H3K4me1), a post-translational histone modification enriched at active and poised cis-regulatory regions, in IFN?, Il-1?, and TNF? treated pancreatic islets. We identified 96,721 putative cis-regulatory loci, of which 3,590 were generated de novo, 3,204 had increased H3K4me1, and 5,354 had decreased H3K4me1 in IFN?, Il-1?, and TNF? exposed islets. Roughly 10% of the de novo and increased regions were enriched for the repressive histone modification histone 3 lysine 27 trimethylation (H3K27me3) in untreated cells, and these were frequently associated with chemokine genes. We show that IFN?, Il-1?, and TNF? exposure overcomes this repression and induces chemokine gene activation in as little as three hours, and that this expression persists for days in absence of continued IFN?, Il-1?, and TNF? exposure. We implicate trithorax group (TrxG) complexes as likely players in the conversion of these repressed loci to an active state. To block the activity of these complexes, we suppressed Wdr5, a core component of the TrxG complexes, and used the H3K27me3 demethylase inhibitor GSK-J4. We show that GSK-J4 is particularly effective in blunting IFN?, Il-1?, and TNF?-induced chemokine gene expression in ?-cells; however, it induced significant islet-cell apoptosis and ?-cell dysfunction. Wdr5 suppression also reduced IFN?, Il-1?, and TNF? induced chemokine gene expression in ?-cells without affecting islet-cell survival or ?-cell function after 48hrs, but did begin to increase islet-cell apoptosis and ?-cell dysfunction after four days of treatment. Taken together these data suggest that the TrxG complex is potentially a viable target for preventing cytokine induced chemokine gene expression in ?-cells.
