Ontology highlight
ABSTRACT:
SUBMITTER: Bednar D
PROVIDER: S-EPMC4631455 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Bednar David D Beerens Koen K Sebestova Eva E Bendl Jaroslav J Khare Sagar S Chaloupkova Radka R Prokop Zbynek Z Brezovsky Jan J Baker David D Damborsky Jiri J
PLoS computational biology 20151103 11
There is great interest in increasing proteins' stability to enhance their utility as biocatalysts, therapeutics, diagnostics and nanomaterials. Directed evolution is a powerful, but experimentally strenuous approach. Computational methods offer attractive alternatives. However, due to the limited reliability of predictions and potentially antagonistic effects of substitutions, only single-point mutations are usually predicted in silico, experimentally verified and then recombined in multiple-po ...[more]