Ontology highlight
ABSTRACT:
SUBMITTER: Capone S
PROVIDER: S-EPMC4632689 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Capone Simona S Ćorajević Lejla L Bonifert Günther G Murth Patrick P Maresch Daniel D Altmann Friedrich F Herwig Christoph C Spadiut Oliver O
International journal of molecular sciences 20150924 10
Horseradish peroxidase (HRP), conjugated to antibodies and lectins, is widely used in medical diagnostics. Since recombinant production of the enzyme is difficult, HRP isolated from plant is used for these applications. Production in the yeast Pichia pastoris (P. pastoris), the most promising recombinant production platform to date, causes hyperglycosylation of HRP, which in turn complicates conjugation to antibodies and lectins. In this study we combined protein and strain engineering to obtain ...[more]