Proteomics

Dataset Information

0

Engineered Pichia pastoris LC-MSMS


ABSTRACT: Samples of PCH, LH73H and LH119H were cultivated according to the culture method of shake flask cultivation and then collected for protein extraction.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Fungi Komagataella Pastoris (strain Gs115 / Atcc 20864) (yeast) (pichia Pastoris)

TISSUE(S): Yeast

SUBMITTER: Linhui Gao  

LAB HEAD: Yongjin J. Zhou

PROVIDER: PXD048269 | Pride | 2024-08-10

REPOSITORIES: Pride

Dataset's files

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Publications

Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis.

Gao Linhui L   Hou Rui R   Cai Peng P   Yao Lun L   Wu Xiaoyan X   Li Yunxia Y   Zhang Lihua L   Zhou Yongjin J YJ  

JACS Au 20240429 7


Microbial metabolic engineering provides a feasible approach to sustainably produce advanced biofuels and biochemicals from renewable feedstocks. Methanol is an ideal feedstock since it can be massively produced from CO<sub>2</sub> through green energy, such as solar energy. However, engineering microbes to transform methanol and overproduce chemicals is challenging. Notably, the microbial production of isoprenoids from methanol is still rarely reported. Here, we extensively engineered <i>Pichia  ...[more]

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