Ontology highlight
ABSTRACT:
SUBMITTER: Shang R
PROVIDER: S-EPMC4633630 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Shang Renfu R Zhang Fengjuan F Xu Beiying B Xi Hairui H Zhang Xue X Wang Weihua W Wu Ligang L
Nature communications 20151012
Short-hairpin RNAs (shRNAs) are widely used to produce small-interfering RNAs (siRNAs) for gene silencing. Here we design an alternative siRNA precursor, named single-stranded, Argonaute 2 (Ago2)-processed interfering RNA (saiRNA), containing a 16-18 bp stem and a loop complementary to the target transcript. The introduction of a self-cleaving ribozyme derived from hepatitis delta virus to the 3' end of the transcribed saiRNA dramatically improves its silencing activity by generating a short 3' ...[more]