Ontology highlight
ABSTRACT:
SUBMITTER: Tsankov AM
PROVIDER: S-EPMC4636964 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Tsankov Alexander M AM Akopian Veronika V Pop Ramona R Chetty Sundari S Gifford Casey A CA Daheron Laurence L Tsankova Nadejda M NM Meissner Alexander A
Nature biotechnology 20151026 11
Research on human pluripotent stem cells has been hampered by the lack of a standardized, quantitative, scalable assay of pluripotency. We previously described an assay called ScoreCard that used gene expression signatures to quantify differentiation efficiency. Here we report an improved version of the assay based on qPCR that enables faster, more quantitative assessment of functional pluripotency. We provide an in-depth characterization of the revised signature panel (commercially available as ...[more]