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A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells.


ABSTRACT: Research on human pluripotent stem cells has been hampered by the lack of a standardized, quantitative, scalable assay of pluripotency. We previously described an assay called ScoreCard that used gene expression signatures to quantify differentiation efficiency. Here we report an improved version of the assay based on qPCR that enables faster, more quantitative assessment of functional pluripotency. We provide an in-depth characterization of the revised signature panel (commercially available as the TaqMan hPSC Scorecard Assay) through embryoid body and directed differentiation experiments as well as a detailed comparison to the teratoma assay. We further show that the improved ScoreCard enables a wider range of applications, such as screening of small molecules, genetic perturbations and assessment of culture conditions. Our approach can be extended beyond stem cell applications to characterize and assess the utility of other cell types and lineages.

SUBMITTER: Tsankov AM 

PROVIDER: S-EPMC4636964 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells.

Tsankov Alexander M AM   Akopian Veronika V   Pop Ramona R   Chetty Sundari S   Gifford Casey A CA   Daheron Laurence L   Tsankova Nadejda M NM   Meissner Alexander A  

Nature biotechnology 20151026 11


Research on human pluripotent stem cells has been hampered by the lack of a standardized, quantitative, scalable assay of pluripotency. We previously described an assay called ScoreCard that used gene expression signatures to quantify differentiation efficiency. Here we report an improved version of the assay based on qPCR that enables faster, more quantitative assessment of functional pluripotency. We provide an in-depth characterization of the revised signature panel (commercially available as  ...[more]

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