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Differentiation of human pluripotent stem cells into functional airway basal stem cells.

ABSTRACT: Airway basal cells play an essential role in the maintenance of the airway epithelium. Here, we provide a detailed directed differentiation protocol to generate ''induced basal cells (iBCs)'' from human pluripotent stem cells. iBCs recapitulate biological and functional properties of airway basal cells including mucociliary differentiation in vitro or in vivo in tracheal xenografts, facilitating the study of inherited and acquired diseases of the airway, as well as potential use in regenerative medicine. For complete details on the use and execution of this protocol, please refer to Hawkins et al. (2021).

SUBMITTER: Suzuki S

PROVIDER: S-EPMC8322461 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Differentiation of human pluripotent stem cells into functional airway basal stem cells.

Suzuki Shingo S Hawkins Finn J FJ Barillà Cristina C Beermann Mary Lou ML Kotton Darrell N DN Davis Brian R BR

STAR protocols 20210720 3

Airway basal cells play an essential role in the maintenance of the airway epithelium. Here, we provide a detailed directed differentiation protocol to generate ''induced basal cells (iBCs)'' from human pluripotent stem cells. iBCs recapitulate biological and functional properties of airway basal cells including mucociliary differentiation <i>in vitro</i> or <i>in vivo</i> in tracheal xenografts, facilitating the study of inherited and acquired diseases of the airway, as well as potential use in ...[more]

PMID: 34355203

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