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Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells.


ABSTRACT: Neuroblastoma (NB) is the most common extracranial solid tumor that originates from multipotent neural crest cells. NB cell populations that express embryonic stem cell-associated genes have been identified and shown to retain a multipotent phenotype. However, whether somatic reprogramming of NB cells can produce similar stem-cell like populations is unknown. Here, we sought to reprogram NB cell lines using an integration-free Sendai virus vector system. Of four NB cell lines examined, only SH-IN cells formed induced pluripotent stem cell-like colonies (SH-IN 4F colonies) at approximately 6 weeks following transduction. These SH-IN 4F colonies were alkaline phosphatase-positive. Array comparative genomic hybridization analysis indicated identical genomic aberrations in the SH-IN 4F cells as in the parental cells. SH-IN 4F cells had the ability to differentiate into the three embryonic germ layers in vitro, but rather formed NBs in vivo. Furthermore, SH-IN 4F cells exhibited resistance to cisplatin treatment and differentiated into endothelial-like cells expressing CD31 in the presence of vascular endothelial growth factor. These results suggest that SH-IN 4F cells are partially reprogrammed NB cells, and could be a suitable model for investigating the plasticity of aggressive tumors.

SUBMITTER: Islam SM 

PROVIDER: S-EPMC4638011 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells.

Islam S M Rafiqul SM   Suenaga Yusuke Y   Takatori Atsushi A   Ueda Yasuji Y   Kaneko Yoshiki Y   Kawana Hidetada H   Itami Makiko M   Ohira Miki M   Yokoi Sana S   Nakagawara Akira A  

Cancer science 20150818 10


Neuroblastoma (NB) is the most common extracranial solid tumor that originates from multipotent neural crest cells. NB cell populations that express embryonic stem cell-associated genes have been identified and shown to retain a multipotent phenotype. However, whether somatic reprogramming of NB cells can produce similar stem-cell like populations is unknown. Here, we sought to reprogram NB cell lines using an integration-free Sendai virus vector system. Of four NB cell lines examined, only SH-I  ...[more]

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