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Experimental study of the protective effects of SYVN1 against diabetic retinopathy.

ABSTRACT: Genetic factors play an important role in the pathogenesis of diabetic retinopathy (DR). While many studies have focused on genes that increase susceptibility to DR, herein, we aimed to explore genes that confer DR resistance. Previously, we identified Hmg CoA reductase degradation protein 1 (SYVN1) as a putative DR protective gene via gene expression analysis. Transgenic mice overexpressing SYVN1 and wild-type (WT) mice with streptozotocin-induced diabetes were used in this experiment. Retinal damage and vascular leakage were investigated 6 months after induction of diabetes by histopathological and retinal cell apoptosis analyses and by retinal perfusion of fluorescein isothiocyanate-conjugated dextran. Compared with diabetic WT mice, diabetic SYVN1 mice had significantly more cells and reduced apoptosis in the retinal ganglion layer. Retinal vascular leakage was significantly lower in diabetic SYVN1 mice than in diabetic WT mice. The expression levels of endoplasmic reticulum (ER) stress-related, pro-inflammatory, and pro-angiogenic genes were also analyzed. Lower expression levels were observed in diabetic SYVN1 mice than in WT controls, suggesting that SYVN1 may play an important role in inhibiting ER stress, chronic inflammation, and vascular overgrowth associated with DR. Thus, these results strongly supported our hypothesis that SYVN1 confers DR resistance.

SUBMITTER: Yang S

PROVIDER: S-EPMC4642554 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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Experimental study of the protective effects of SYVN1 against diabetic retinopathy.

Yang Shuo S He Heng H Ma Qi Si QS Zhang Yong Y Zhu Ying Y Wan Xing X Wang Feng Wen FW Wang Shuai Shuai SS Liu Lei L Li Bin B

Scientific reports 20150911

Genetic factors play an important role in the pathogenesis of diabetic retinopathy (DR). While many studies have focused on genes that increase susceptibility to DR, herein, we aimed to explore genes that confer DR resistance. Previously, we identified Hmg CoA reductase degradation protein 1 (SYVN1) as a putative DR protective gene via gene expression analysis. Transgenic mice overexpressing SYVN1 and wild-type (WT) mice with streptozotocin-induced diabetes were used in this experiment. Retinal ...[more]

PMID: 26358086

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Project description:Background:Male hypogonadism is associated with type II diabetes mellitus due to testicular dysfunction. Medicinal plants have received considerable attention for the management of diabetes and its complications. Objective:The aim of present study was to evaluate the anti-diabetic and protective influence of Crocin on testopathy in diabetic rats. Materials and Methods:In this experimental study, type II of diabetes mellitus was induced by high-fat diet and low dose of streptozotocin. Male Wistar rats (8 weeks, 150-200 gr, 18 rats; n= 6 per group) were divided into a control group (standard diet), diabetic group (streptozotocin+high-fat diet), and treatment group (High-fat diet+streptozotocin+Crocin at 20 mg/kg/day, i.p. for 60 days). After 60 days, animals were euthanized, testis and epididymis were dissected, and weights of testes and sperm count were analyzed. Hematoxylin-eosin-stained was done for histopathological examination. Blood samples were collected for the assessment of serum glucose and cholesterol. Results:High-fat diet and streptozotocin significantly increased the serum glucose and cholesterol levels as compared to the control group (p ? 0. 001). Moreover, there was a significant decrease in the weight of right (p= 0.008) and left testes (p ? 0. 001) and also the total sperm count (p= 0.023) in the diabetic group compared with the control group. Current results also identified that type II diabetes mellitus induced degeneration in the morphology of seminiferous tubules. Application of Crocin could significantly decrease serum glucose and cholesterol levels (p= 0.003). Furthermore, Crocin treatment significantly increased the weight of the right (p= 0.026) and left (p= 0.014) testes and total sperm count (p= 0.000). Also, Crocin could attenuate the pathological changes of the testes in the treatment group. Conclusion:Present findings concluded that Crocin treatment improved diabetic testopathy and impairment of seminiferous tubules induced by high-fat diet and streptozotocin.

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Experimental study of the protective effects of SYVN1 against diabetic retinopathy.

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Experimental study of the protective effects of SYVN1 against diabetic retinopathy.

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