Predictive Spatiotemporal Manipulation of Signaling Perturbations Using Optogenetics.

ABSTRACT: Recently developed optogenetic methods promise to revolutionize cell biology by allowing signaling perturbations to be controlled in space and time with light. However, a quantitative analysis of the relationship between a custom-defined illumination pattern and the resulting signaling perturbation is lacking. Here, we characterize the biophysical processes governing the localized recruitment of the Cryptochrome CRY2 to its membrane-anchored CIBN partner. We develop a quantitative framework and present simple procedures that enable predictive manipulation of protein distributions on the plasma membrane with a spatial resolution of 5 μm. We show that protein gradients of desired levels can be established in a few tens of seconds and then steadily maintained. These protein gradients can be e

SUBMITTER: Valon L 

PROVIDER: S-EPMC4643200 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature