High-throughput mRNA and miRNA profiling of epithelial-mesenchymal transition in MDCK cells.

ABSTRACT:

Background

Epithelial-mesenchymal transition (EMT) is an important process in embryonic development, especially during gastrulation and organ formation. Furthermore EMT is widely observed in pathological conditions, e.g., fibrosis, tumor progression and metastasis. Madin-Darby Canine Kidney (MDCK) cells are widely used for studies of EMT and epithelial plasticity. MDCK cells show an epithelial phenotype, while oncogenic Ras-transformed MDCK (MDCK-Ras) cells undergo EMT and show a mesenchymal phenotype.

Methods

RNA-Seq and miRNA-Seq analyses were performed on MDCK and MDCK-Ras cells. Data were validated by qRT-PCR. Gene signature analyses were carried out to identify pathways and gene ontology terms. For selected miRNAs target prediction was performed.

Results

With RNA

SUBMITTER: Shukla P 

PROVIDER: S-EPMC4647640 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature