High-throughput transcriptomic and proteomic profiling of mesenchymal-amoeboid transition in 3D collagen.
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ABSTRACT:
SUBMITTER: Cermak V
PROVIDER: S-EPMC7253430 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Publications
High-throughput transcriptomic and proteomic profiling of mesenchymal-amoeboid transition in 3D collagen.
Čermák Vladimír V Gandalovičová Aneta A Merta Ladislav L Harant Karel K Rösel Daniel D Brábek Jan J
Scientific data 20200527 1
The plasticity of cancer cell invasion represents substantial hindrance for effective anti-metastatic therapy. To better understand the cancer cells' plasticity, we performed complex transcriptomic and proteomic profiling of HT1080 fibrosarcoma cells undergoing mesenchymal-amoeboid transition (MAT). As amoeboid migratory phenotype can fully manifest only in 3D conditions, all experiments were performed with 3D collagen-based cultures. Two previously described approaches to induce MAT were used: ...[more]
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