Ontology highlight
ABSTRACT:
SUBMITTER: Liu J
PROVIDER: S-EPMC4652282 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Scientific reports 20151119
Fluorophore labeling of proteins while preserving native functions is essential for bulk Förster resonance energy transfer (FRET) interaction and single molecule imaging analysis. Here we describe a versatile, efficient, specific, irreversible, gentle and low-cost method for labeling proteins with fluorophores that appears substantially more robust than a similar but chemically distinct procedure. The method employs the controlled enzymatic conversion of a central Cys to a reactive formylglycine ...[more]