Ontology highlight
ABSTRACT:
SUBMITTER: Uttamapinant C
PROVIDER: S-EPMC2890758 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Uttamapinant Chayasith C White Katharine A KA Baruah Hemanta H Thompson Samuel S Fernández-Suárez Marta M Puthenveetil Sujiet S Ting Alice Y AY
Proceedings of the National Academy of Sciences of the United States of America 20100607 24
Biological microscopy would benefit from smaller alternatives to green fluorescent protein for imaging specific proteins in living cells. Here we introduce PRIME (PRobe Incorporation Mediated by Enzymes), a method for fluorescent labeling of peptide-fused recombinant proteins in living cells with high specificity. PRIME uses an engineered fluorophore ligase, which is derived from the natural Escherichia coli enzyme lipoic acid ligase (LplA). Through structure-guided mutagenesis, we created a mut ...[more]